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说明书

 

TPO,甲状腺过氧化物酶

 

 

 

 

4TP15

 

 

 

6H7

IgG1

EIA


TPO28

IgG1

EIA  WB

TPO34

IgG1

EIA, WB

TPO35

IgG1

EIA


抗原

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说明书

TPO,甲状腺过氧化物酶

8T58

>90%

人甲状腺


TPO,甲状腺过氧化物酶,重组

8RTP0

>95%

重组


重组人甲状腺过氧化物酶


Thyroid Peroxidase (TPO) is an integralapical membrane glycoprotein of thyroid follicular cells, which is responsiblefor the tyrosine residues in thyroglobulin, leading to thyroid hormonegeneration (Ruf and Carayon, 2006). In membrane TPO is found as a homodimerwith subunits of approximately 100 kDa molecular weight (Baker et al. 1994).The anti-thyroid peroxidase autoantibodies are the most frequently representedautoantibodies in the sera of patients suffering from autoimmune thyroiddisease; they are present in 90% of Hashimoto’s thyroiditis and 75% of Graves’disease patients (Mariotti et al. 1990). Thus immunoassays for quantificationof anti-TPO autoantibodies are widely used in clinical practice.

 

For many years human native TPO, purifiedfrom thyroid glands, has been used as a key component - an antigen - in suchassays. Studies in early 90s revealed that soluble extracellular domain ofrecombinant human thyroid peroxidase (rTPO) produced in insect cells has immunochemicalproperties similar to native human thyroid peroxidase (Haubruck et al. 1993).The technical limitations in the production of bulk amounts of rTPO haveimposed some restrictions on the wide rTPO utilization in assays.

 

Recombinant human TPO offered by HyTest hasimmunochemical properties similar to the native antigen purified from humanthyroid glands. It can be utilized as an antigen in the assays for thedetection of human TPO-specific autoantibodies in the blood of the patientswith autoimmune thyroid diseases.

 

HyTest offers recombinant human TPOexpressed in insect cells as soluble extracellular domain of human TPO (aminoacid residues 19-846). Calculated molecular weight of the protein 91981 Da.Recombinant human TPO does not contain any tags. The protein is purified tohomogeneity using specific monoclonal antibody-based affinity and ion exchangechromatography methods (Fig. 1). Two visible protein bands (in the region of100-110 kDa) on the gel after SDS-PAGE gel electrophoresis belong to TPO sinceboth react with specific monoclonal antibodies in Western blotting. Theelectrophoretic heterogeneity of TPO is apparently caused by differentialglycosylation of the protein. The identity of TPO was also confirmed by MALDImass-spectrometry.


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